NOTES ON BLOOD CULTURE TECHNIQUE

Simplified lysed-blood culture technique.

A blood culture system was developed in which a lysing agent (either Tween 20, one of several other polyoxyethylene adducts, digitonin, or Triton X-100) is added to the blood culture medium. Of 33 Triton compounds, 9 lysed human blood, as did 7 of 21 polyoxyethylene compounds and digitonin, all at a concentration of 0.05%. Under the specific test conditions, three of the hemolytic polyoxyethyle...

Practical aerobic membrane filtration blood culture technique: clinical blood culture trial.

During the course of preliminary clinical trials of an improved membrane filter blood culture system, filter plugging produced by a gelling of the lysing solution was observed when the patients had high leukocyte counts. A solution of streptokinase-streptodornase (Varidase, Lederle Laboratories) dissolved or prevented the gel and permitted rapid filtration without plugging. With streptokinase-s...

Notes on a Simple Technique of Splenic Culture at the Post-Mortem Table

The following technique has proved useful in the diagnosis of the enteric group of fevers and of septicaemia of various origin. The advantages are : increased probability of obtaining the organism, and adaptability to adverse circumstances, such as lack of facilities for heartblood culture. Equipment : one or two throat swabs, one scalpel and one spirit burner. Post mortem is to be carried out ...

LECTURE NOTES ON The Star Complement Technique

Practical aerobic membrane filtration blood culture technique: development of procedure.

The advantages of a membrane filter system for blood culturing have been realized for many years. Lysing of the blood prior to filtration is a convenient way to proceed, but previously described lysing procedures result in loss of certain organisms, particularly gram-negative bacilli. Four concentrations of Triton X-100 and sodium carbonate were studied in vitro, and their lysing and antibacter...